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Mycoplasma detection
Mycoplasma Elimination Reagent(100×)

Mycoplasma Elimination Reagent(100×)

  • Product detail
Product information
The product name The article number Packing specification unit List price
Mycoplasma Elimination Reagent(100×) DIB4021-10ML 10×1 mL package 2000

Product introduction
It is a combination of quinolones and tetracycline, which interfere with DNA replication and protein synthesis of mycoplasma, respectively, so as to effectively eliminate mycoplasma. This product has low toxicity to eukaryotic cells and can completely remove infected mycoplasma in about 10 days. After normal medium is replaced, the cells are in good condition and there is no repositive phenomenon in continuous multi-generation culture.

Storage and transportation
Wet ice transportation; Stored at -20℃ to avoid repeated freeze-thaw, valid for 12 months.

Kit composition



Mycoplasma Elimination Reagent(100×)

10×1 mL

Product specification

1Copy of the


1. Add 1 mL MycoplasmaElimination Reagent (100×) into 100 mL cell medium (containing serum, without double antibody), mix thoroughly to obtain the medium containing MycoplasmaElimination Reagent;
2. Replace medium containing Mycoplasma Elimination Reagent for routine cell culture when Mycoplasma infected cells are subcultured or fluid is changed;
3. After one week of culture, some cells were taken and inoculated into 6-well plates (or other culture vessels of other specifications), and the normal complete medium was replaced. The remaining cells were cultured in medium containing Mycoplasma Elimination Reagent.
4. Cells in 6-well plates were cultured in normal complete medium for 2-3 days, and tested for mycoplasma when the confluence of cells reached over 90% (DIB1915, DIB1916, DIB1917);
5. Perform the following operations based on the test results:
A) Positive results: Repeat steps 3-4 until the test results are negative.
B) Negative results: The cell group containing Mycoplasma Elimination Reagent medium was replaced with normal complete medium, and Mycoplasma was detected after a period of culture; A negative retest indicates completion of clearance.
Matters needing attention
1. This product has been tested in a variety of mycoplasma infected cell lines, but different cell lines have different sensitivity to the reagent. If the cytotoxicity is too great or the clearance effect is not good, the solubility of the product can be adjusted appropriately, or other scavengers can be used (DIB4022, DIB4023 are recommended).
2. There are many ways for cells to be infected with mycoplasma, so it is recommended to add mycoplasma prevention reagent (DIB4023 is recommended) in daily culture.
3. For cells that have been determined to be contaminated with Mycoplasma, try to use a separate ultra-clean table during operation to avoid cross-contamination.
4. For your safety and health, please wear lab coat and disposable gloves.

Attached table: Characteristics of mycoplasma removal products

The product name

Mycoplasma Elimination Reagent(100×)

Mycoplasma Elimination Reagent Plus(100×)

Mycoplasma Elimination Reagent(SweMyco-1+2)(100×)

Product item no





Quinolones, tetracycline

Quinolones, tetracycline, macrolides

Quinolones, tetracycline, macrolides

The relative toxicity

In the



Clearance speed

About ten days

5 to 7 days

7 days or so

Ease of use



More complicated

Products for scientific research purposes only, not for clinical diagnosis!

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