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Tag antibody
GFP-tag(ME11)Mouse Monoclonal Antibody

GFP-tag(ME11)Mouse Monoclonal Antibody

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GFP label mouse monoclonal antibody manual
GFP-tag(ME11)Mouse Monoclonal Antibody

 
Product number          DIB1013         DIB1013L
specification                 100ul             1ml  
Product Name: Anti-GFP Tag Mouse Monoclonal Antibody
Antibody type: mouse IgG1
Clone number: ME11
Concentration: 1mg/ml
Immunogen: GFP full-length protein as antigen.
Product description: GFP (Green Fluorescent Protein) is a luminescent protein found in jellyfish. It is composed of 238 amino acids and has a molecular weight of 26KD.The green fluorescent protein gene is very stable after being transferred into host cells and has no effect on the physiology of most hosts. It is a commonly used reporter gene.The company's GFP antibody is a highly purified monoclonal antibody that can highly specifically recognize the GFP sequence in the fusion protein.
Recommended dilution: Western blot (WB): 1:1000—1:10000
                                         Immunofluorescence (IF): 1:100—1:1000
                                         Immunoprecipitation (IP): 1:100—1:1000
Solution composition: 0.01M PBS (PH7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Storage conditions: Store at -20°C.
Validity period: 12 months.
Matters needing attention:
1. Please choose to use anti-mouse secondary antibody, such as goat anti-mouse IgG (H+L)-HRP (DIB3006).
2. Please determine the best antibody dilution according to the experiment.
3. This product is only used for scientific research and cannot be used for clinical diagnosis.
Relevant data:  
      Use GFP protein to detect GFP-tag antibody (DIB1013 ), and the amount of protein loaded per hole is 100ng.The antibody was diluted 1:5000 (1), 1:10000 (2), and 1:20000 (3).
 
   
      Detect the GFP-tag antibody (DIB1013 ) with GFP-containing protein, the antibody is diluted 1:5000, and the amount of protein loaded per well is 20ng (1), 10ng (2), 5ng (3), 2.5ng (4), 1.2 ng(5), 0.6ng(6).
 
 

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